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1.1 Microbiological test methods present challenges that are unique relative to chemical or physical parameters, because microbes proliferate, die off and continue to be metabolically active in samples after those samples have been drawn from their source.
1.1.1 Microbial activity depends on the presence of available water. Consequently, the detection and quantification of microbial contamination in fuels and lubricants is made more complicated by the general absence of available water from these fluids.
1.1.2 Detectability depends on the physiological state and taxonomic profile of microbes in samples. These two parameters are affected by various factors that are discussed in this guide, and contribute to microbial data variability.
1.2 This guide addresses the unique considerations that must be accounted for in the design and execution of interlaboratory studies intended to determine the precision of microbiological test methods designed to quantify microbial contamination in fuels, lubricants and similar low water-content (water activity <0.8) fluids.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
Energy Institute StandardIP 385 Viable aerobic microbial content of fuels and fuel components boiling below 90C--Filtration and culture method
D156 Test Method for Saybolt Color of Petroleum Products (Saybolt Chromometer Method)
D1129 Terminology Relating to Water
D4012 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
D4175 Terminology Relating to Petroleum, Petroleum Products, and Lubricants
D6300 Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
D6469 Guide for Microbial Contamination in Fuels and Fuel Systems
D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels--Filtration and Culture Procedures
D7463 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
D7687 Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration
E1259 Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390C
E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ICS Number Code 07.100.01 (Microbiology in general); 75.100 (Lubricants, industrial oils and related products)
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ASTM D7847-12e1, Standard Guide for Interlaboratory Studies for Microbiological Test Methods, ASTM International, West Conshohocken, PA, 2012, www.astm.orgBack to Top