ASTM D7304 - 06

    Standard Test Method for Determination of Denatonium Ion in Engine Coolant by HPLC

    Active Standard ASTM D7304 | Developed by Subcommittee: D15.04

    Book of Standards Volume: 15.05


    Buy Standard (PDF)
    5 pages
    $42.00
    Buy Standard (Print)
    5 pages
    $42.00


    ASTM D7304

    Significance and Use

    This test method provides for the qualitative and quantitative determination of denatonium ion in engine coolant in milligrams per liter to low percent range and requires approximately 100 mL per test, with results available in less than 30 min. Acceptable levels of denatonium vary with manufacturers blending specifications and applicable minimum or maximum industry and state specifications. Typically specification ranges from 30 to 50 ppm denatonium ion.

    1. Scope

    1.1 This test method covers the chemical analysis of engine coolant for denatonium ion by high-performance liquid chromatography (HPLC).

    1.2 This test method is applicable to both new and used coolants.

    1.3 Coelution of other ions may cause interferences in the detection of the denatonium cation. In the case of unfamiliar formulations, identification verification should be performed by either or both fortification and dilution of the sample matrix with denatonium ion..

    1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

    This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1176 Practice for Sampling and Preparing Aqueous Solutions of Engine Coolants or Antirusts for Testing Purposes

    D1193 Specification for Reagent Water


    ICS Code

    ICS Number Code 71.100.45 (Refrigerants and antifreezes)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/D7304-06

    ASTM International is a member of CrossRef.

    ASTM D7304

    Citing ASTM Standards
    Back to Top