ASTM D5712 - 10

    Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method

    Active Standard ASTM D5712 | Developed by Subcommittee: D11.40

    Book of Standards Volume: 09.02


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    ASTM D5712

    Significance and Use

    This test method, for the determination of protein levels in NR, is primarily intended to test NR materials for residual protein content. It is assumed that all who use this test method will be trained analysts capable of performing common laboratory procedures skillfully and safely. It is expected that work will be performed in a properly equipped laboratory.

    1. Scope

    1.1 This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with natural rubber (NR) and its products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

    1.2 For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm2 NR specimen.

    1.3 The test method is designed to be accurate and compatible with the industrial environment.

    1.4 Steps are included in this test method to minimize the effects of interfering substances.

    1.5 It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this test method.

    1.6 This test method has not been validated for use with lubricated products such as condoms. Condoms with different lubricants as typically marketed, have not been tested in an ASTM ILS to determine if, and if so to what degree, the lubricant interferes with the assay.

    1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D3577 Specification for Rubber Surgical Gloves

    D3578 Specification for Rubber Examination Gloves

    D4483 Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries


    ICS Code

    ICS Number Code 83.040.10 (Latex and raw rubber)

    UNSPSC Code

    UNSPSC Code 13101500(Natural rubber)


    DOI: 10.1520/D5712-10

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