ASTM D5465 - 93(2012)

    Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

    Active Standard ASTM D5465 | Developed by Subcommittee: D19.24

    Book of Standards Volume: 11.02


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    Significance and Use

    These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.

    Section
    ACounting Colonies on Membrane Filters4
    BCounting Colonies on Pour Plates5
    CCounting Colonies on Spread Plates6

    The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.

    1. Scope

    1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.

    1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    ICS Code

    ICS Number Code 07.100.20 (Microbiology of water)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/D5465-93R12

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