ASTM D5259 - 92(2012)

    Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure

    Active Standard ASTM D5259 | Developed by Subcommittee: D19.24

    Book of Standards Volume: 11.02


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    Significance and Use

    The enterococci are indicators of the bacteriological quality for potable water, shellfish growing waters, ambient, and recreational waters. A direct relationship between swimming, associated gastroenteritis, and enterococci has been established through epidemiological studies and marine and fresh water bathing beaches. These studies have led to the development of criteria that can be used to establish bathing water standards based on established health-water quality relationships.

    Since small or large volumes of water or dilutions thereof, can be analyzed by the membrane filter technique, a wide range of levels of enterococci in water can be enumerated and detected.

    1. Scope

    1.1 This test method covers a membrane filter (MF) procedure for the detection and enumeration of the enterococci bacteria in water. The enterococci, which include Entero-coccus faecalis (E. faecalis), E. faecium, and their varieties are commonly found in the feces of humans and other warm-blooded animals. Although some strains are ubiquitous and not related to fecal pollution, enterococci in water are an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit is one colony forming unit (CFU)/volume filtered.

    1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user's responsibility to ensure the validity of this test method for waters of untested types.

    1.3 The values stated in SI units are to be regarded as the standard.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D3370 Practices for Sampling Water from Closed Conduits

    D3870 Practice for Establishing Performance Characteristics for Colony Counting Methods in Microbiology


    ICS Code

    ICS Number Code 13.060.70 (Examination of water for biological properties)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/D5259-92R12

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