ASTM D5246 - 13

    Standard Test Method for Isolation and Enumeration of Pseudomonas aeruginosa from Water

    Active Standard ASTM D5246 | Developed by Subcommittee: D19.24

    Book of Standards Volume: 11.02


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    Significance and Use

    5.1 Pseudomonas aeruginosa is an opportunistic pathogen, and has been linked as the causative agent of numerous infections that may be transmitted through a contaminated water supply to a susceptible host.

    Note 1Fecal waste is >95 % E. coli which is found in humans and warm bloodied animals.

    5.2 The membrane filtration procedure described is a rapid and reliable test method of detecting P. aeruginosa in water.

    1. Scope

    1.1 The test method covers the isolation and enumeration of Pseudomonas aeruginosa. Testing was performed on spiked reagent grade water samples.

    1.2 It is the user’s responsibility to ensure the validity of this method for surface waters, ground waters, recreational waters fresh and marine), wastewaters.

    1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 10.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D2777 Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water

    D3370 Practices for Sampling Water from Closed Conduits


    ICS Code

    ICS Number Code 07.100.20 (Microbiology of water)

    UNSPSC Code

    UNSPSC Code


    Referencing This Standard

    DOI: 10.1520/D5246

    ASTM International is a member of CrossRef.

    Citation Format

    ASTM D5246-13, Standard Test Method for Isolation and Enumeration of Pseudomonas aeruginosa from Water, ASTM International, West Conshohocken, PA, 2013, www.astm.org

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