ASTM D5245 - 92(2012)

    Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses

    Active Standard ASTM D5245 | Developed by Subcommittee: D19.24

    Book of Standards Volume: 11.02


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    Significance and Use

    This practice provides uniform guidance for cleaning the laboratory glassware, plasticware, and equipment used in routine microbiological analyses. However, tests that are extremely sensitive to toxic agents (such as virus assays) may require more stringent cleaning practices.

    1. Scope

    1.1 In microbiology, clean glassware is crucial to ensure valid results. Previously used or new glassware must be thoroughly cleaned. Laboratory ware and equipment that are not chemically clean are responsible for considerable losses in personnel time and supplies in many laboratories. These losses may occur as down time when experiments clearly have been adversely affected and as invalid data that are often attributed to experimental error. Chemical contaminants that adversely affect experimental results are not always easily detected. This practice describes the procedures for producing chemically clean glassware.

    1.2 The values stated in SI units are to be regarded as the standard.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific precautions, see Section 5, 7.3.1, and Note 1 and Note 2.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1193 Specification for Reagent Water


    ICS Code

    ICS Number Code 07.100.01 (Microbiology in general)

    UNSPSC Code

    UNSPSC Code


    Referencing This Standard

    DOI: 10.1520/D5245-92R12

    ASTM International is a member of CrossRef.

    Citation Format

    ASTM D5245-92(2012), Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses , ASTM International, West Conshohocken, PA, 2012, www.astm.org

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