ASTM D5196 - 06(2013)

    Standard Guide for Bio-Applications Grade Water

    Active Standard ASTM D5196 | Developed by Subcommittee: D19.02

    Book of Standards Volume: 11.01


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    ASTM D5196

    Significance and Use

    4.1 The purity of water is relative and is usually characterized by the limits of impurities found in the water as well as by the methods used to prepare and handle the water. Section 7 mentions the suitable methods for water preparation.

    1. Scope

    1.1 This guide is intended to describe the chemical and biological characteristics of water to be used whenever critical purity is essential to the use intended in laboratory Bio-Applications, for example, clinical, pharmaceutical, and biomedical. The importance of such a reagent is often underestimated despite the impact that it can have.

    1.2 This guide is not intended to be used as a reference in preparing water for injectables. Generally, the appropriate use of this guide may include experiments involving tissue culture, chromatography, mass spectrometry, Polymerase Chain Reaction (PCR), DeoxyriboNucleic Acid (DNA) sequencing, DNA hybridization, electrophoresis, molecular biology or analyses where molecular concentrations of impurities may be important.

    1.3 For all the other applications linked to an ASTM method and not bio-sensitive that require purified water, it is recommended that Specification D1193 or Test Method D5127 be consulted.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1125 Test Methods for Electrical Conductivity and Resistivity of Water

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D4453 Practice for Handling of High Purity Water Samples

    D5127 Guide for Ultra-Pure Water Used in the Electronics and Semiconductor Industries

    D5173 Test Method for On-Line Monitoring of Carbon Compounds in Water by Chemical Oxidation, by UV Light Oxidation, by Both, or by High Temperature Combustion Followed by Gas Phase NDIR or by Electrolytic Conductivity

    D5245 Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses

    D5391 Test Method for Electrical Conductivity and Resistivity of a Flowing High Purity Water Sample

    D5542 Test Methods for Trace Anions in High Purity Water by Ion Chromatography

    D5673 Test Method for Elements in Water by Inductively Coupled Plasma--Mass Spectrometry

    D5996 Test Method for Measuring Anionic Contaminants in High-Purity Water by On-Line Ion Chromatography

    F1094 Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method


    ICS Code

    ICS Number Code 71.060.01 (Inorganic chemicals in general)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/D5196-06R13

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