ASTM D4200 - 82(2011)

    Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters

    Active Standard ASTM D4200 | Developed by Subcommittee: D19.08

    Book of Standards Volume: 11.02


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    Significance and Use

    This test method may be applied to determine the suitability of grid-marked membrane filters for use in bacteriological culture techniques for the detection and enumeration of bacterial organisms.

    A particularly sensitive organism and growth conditions have been selected for this test method in order to maximize sensitivity to toxic materials possibly present in the inks used for grid-marking membrane filters.

    1. Scope

    1.1 This test method describes a procedure whereby the user of ink-gridded membrane filters in water quality studies can ascertain whether or not the grid lines are toxic and inhibitory to bacterial growth when the membrane and its entrapped bacteria are incubated on a suitable media.

    1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water


    ICS Code

    ICS Number Code 71.040.20 (Laboratory ware and related apparatus)

    UNSPSC Code

    UNSPSC Code


    Referencing This Standard

    DOI: 10.1520/D4200-82R11

    ASTM International is a member of CrossRef.

    Citation Format

    ASTM D4200-82(2011), Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters, ASTM International, West Conshohocken, PA, 2011, www.astm.org

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