ASTM D4198 - 82(2011)

    Standard Test Methods for Evaluating Absorbent Pads Used with Membrane Filters for Bacteriological Analysis and Growth

    Active Standard ASTM D4198 | Developed by Subcommittee: D19.08

    Book of Standards Volume: 11.02


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    Significance and Use

    These test methods are appropriate for qualifying absorbent pads used with membrane filters for bacteriological enumeration.

    The test methods described are applicable to quality control testing of absorbent pads by the suppliers and users of these pads and to specification testing of absorbent pads intended for use with membrane filters in bacteriological enumeration.

    Other pure culture organisms and their appropriate culture medium may be substituted for the E. coli and M-FC media for specification testing, as required.

    1. Scope

    1.1 These test methods cover the determination of the nutrient-holding capacity and the toxic or nutritive effect on bacterial growth of organisms retained on a membrane filter, when the absorbent pad being tested is used as a nutrient reservoir and medium supply source for the retained bacteria.

    1.2 The tests described are conducted on 47-mm diameter disks, although other size disks may be employed for bacterial culture techniques.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1129 Terminology Relating to Water

    D1193 Specification for Reagent Water

    D3508 Method for Evaluating Water Testing Membrane Filters for Fecal Coliform Recovery (Discontinued 1994)


    ICS Code

    ICS Number Code 07.100.10 (Medical microbiology)

    UNSPSC Code

    UNSPSC Code 41104921(Laboratory membrane filters)


    DOI: 10.1520/D4198-82R11

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