ASTM D2868 - 10

    Standard Test Method for Nitrogen Content (Kjeldahl) and Hide Substance Content of Leather

    Active Standard ASTM D2868 | Developed by Subcommittee: D31.06

    Book of Standards Volume: 15.04


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    Significance and Use

    The nitrogen content as determined by this test method is normally considered to be related to the amount of hide substance (protein fiber) present in the leather sample. A factor of 5.62 is normally used to calculate the hide substance from the nitrogen content.

    The 5.62 factor represents the average result of many analyses of animal hides, but it cannot be considered to be accurate since it varies somewhat from hide to hide of the same type, from type of hide to type of hide, and also with the thickness of hide retained in the final leather (split thickness as compared to original hide thickness). As a result of these variations, the true factor for any given leather may be expected to vary from 5.44 to 5.80 or about ±3 %.

    A given leather sample may contain nitrogenous substances other than hide substance (protein fiber) which will be analyzed for by this test method, such as resins, dyestuffs, etc., that contain nitrogen. Therefore, although this test method is fairly accurate for determining the nitrogen content of leather, its use for determining hide substance may result in large errors.

    The hide substance value derived from this determination has a large bearing on other chemical determinations of a given leather. Any errors, such as those described in 4.1.1 and 4.2, will be carried over into these other analytical calculations.

    1. Scope

    1.1 This test method covers the determination of the nitrogen content of all types of leather, wet blue and wet white. The nitrogen content is used to calculate the hide substance (protein fiber) content of leather, wet blue and wet white.

    Note 1—The original test method for leather was essentially a composite of Method 6441 of Federal Test Method Standard No. 311 and Method B5 of the American Leather Chemists Association.

    Note 2—Melamine, if present in bonded leather, could give an artificially high value for the calculation of protein fiber.

    1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D2813 Practice for Sampling Leather for Physical and Chemical Tests

    D6659 Practice for Sampling and Preparation of Wet Blue for Physical and Chemical Tests

    E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods

    E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method


    ICS Code

    ICS Number Code 59.140.30 (Leather and furs)

    UNSPSC Code

    UNSPSC Code 11162300(Leathers)


    Referencing This Standard

    DOI: 10.1520/D2868-10

    ASTM International is a member of CrossRef.

    Citation Format

    ASTM D2868-10, Standard Test Method for Nitrogen Content (Kjeldahl) and Hide Substance Content of Leather, ASTM International, West Conshohocken, PA, 2010, www.astm.org

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