This test method
This test method is based on “A Procedure and Standards for the Determination of the Biodegradability of Alkyl Benzene Sulfonate and Linear Alkylate Sulfonate” by the Committee on Biodegradation Test Methods of the Soap and the Detergent Association, Journal of the Americal Oil Chemists' Society, Vol 42, 1965, p. 986. covers the determination of the degree of biodegradability of alkylbenzene sulfonates. It serves as an index of the suitability of the sulfonate for general use as a surfactant.
Formerly under the jurisdiction of Committee E47 on Biological Effects and Environmental Fate, this test method was withdrawn in March 2013. This standard is being withdrawn without replacement due to its limited use by industry.
1.1 This test method covers the determination of the degree of biodegradability of alkylbenzene sulfonates. It serves as an index of the suitability of the sulfonate for general use as a surfactant.
1.2 In general, this test method distinguishes between sulfonates in which the alkyl side chains are linear and those in which they are branched, since the former are more readily biodegradable. If the alkylbenzene sulfonate in fully formulated products is to be examined, it must be extracted using the method noted in Annex A1. (See Appendix X1 for data.)
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Material Safety Data Sheets are available for reagents and materials. Review them for hazards prior to usage.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D2330 Test Method for Methylene Blue Active Substances
E1298 Guide for Determination of Purity, Impurities, and Contaminants in Biological Drug Products
E1625 Test Method for Determining Biodegradability of Organic Chemicals in Semi-Continuous Activated Sludge (SCAS)
alkylbenzene sulfonate; biodegradability; confirming test; presumptive test; semicontinuous activated sludge; shake culture; Alkylbenzene compounds; Biodegradation; Shake culture procedure; Sludge;
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Citing ASTM Standards
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