Performance of An Enzyme Electrode Designed for a Sulfide Monitoring Biosensor

Sooknah R, Papavinasam S, Attard M, Revie RW, Gould WD, Dinardo O
(Received 18 May 2007; accepted 22 April 2008)
JOURNAL JAI
Abstract

The construction and evaluation of an enzyme electrode designed for a sulfide monitoring biosensor, are reported in this work. The enzyme, which is immobilized in a carbon (graphite) paste with 1,1′ dimethylferrocene, catalyzes the oxidation of sulfide to sulfur.

Amperometric measurements were carried out at room temperature, and a fixed potential of 0.3 V with reference to a saturated calomel electrode, using Tris-HCl buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration range of 0–4mM, giving an average apparent Michaelis constant of 3mM, and maximum current responses between 0.7 and 3.6 μA. The sensitivity of the electrodes tested ranged from 0.2–0.3 μA mM⁻¹.

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Title: Performance of An Enzyme Electrode Designed for a Sulfide Monitoring Biosensor Symposium: Advances in Electrochemical Techniques for Corrosion Monitoring and Measurement, 2007-5-23
Committee: G01 on Corrosion of Metals