Allele Frequencies for Two New Y-chromosome STR Loci DYS607 in Chinese Han Population (Shanxi Area)
Zhang, GQ
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.

Yun, KM
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.

Zhang, XY
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.

Wang, Y
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.

Wang, YY
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi, P. R. China.

(Received 22 November 2003; Accepted 1 December 2003)

Abstract
The whole blood samples of 145 unrelated males of Chinese Han ethnic group who live in Shanxi province, northern of China, were collected and anticoagulant with EDTA. DNA was extracted by the phenol-chloroform method (1). PCR reactions were accomplished in 25 µL reaction mixture, containing 50-100 ng human genome DNA, 200 µM dNTP, 1U Taq polymerase (Sangon, BBI-Canada), 1x buffer, 1.5 mM MgC1&sub2;, and 0.3 µM each primer. The sequences of primers are according with GDB (www.gdb.org). The PCR amplifications of the two STR loci were carried out in a T1 Thermocycler (Biometra, German) with pre-denaturing for 3 min at 95°C, 29 cycles of denaturing for 50s at 94°C, annealing for 50 s at 62°C and extension for 50 s at 72°C. The PCR products were analyzed on a denaturing vertical polyacrylamide gel and visualized by silver staining (2). The alleles were determined by sequencing of PCR products. The nomenclature for the alleles is based on the number of repeat units according to recommendation of the DNA commission of the International Society of Forensic Genetics (3). Statistical analysis were according to Hou's method (4).

Keywords:
allele frequencies, Chinese Han population, DNA typing, DYS598, DYS607, forensic science, Shanxi area, short tandem repeats

Paper ID: JFS2003297
DOI: 10.1520/JFS2003297

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Author Zhang GQ, Yun KM, Zhang XY, Wang Y, Wang YY Title Allele Frequencies for Two New Y-chromosome STR Loci DYS607 in Chinese Han Population (Shanxi Area) Symposium , Committee on