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Allele frequency distribution for 10 STR loci in the Malay population of Malaysia
Panneerchelvam, S School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Haslindawaty, N School of Medical Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Ravichandran, M School of Medical Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Norazmi, MN School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Zainuddin, ZF School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Abstract
DNA data base was obtained from 100 unrelated random Malay individuals in Malaysia. The DNA was extracted by the salting out procedure (1), 20 ng target DNA was co-amplified using the commercial typing kits, Promega Geneprint™ STR multiplex (CTT, FFv, and STR III) and monoplex (LPL) systems, according to the manufacturer's instructions. Assignment of alleles was made by visual comparison between the commercially supplied reference allelic ladders and the amplified samples at the corresponding locus. Data were analyzed as per the methods already reported (2-4). No deviations from equilibrium were observed. The power of discrimination ranges from 0.7298 to 0.9311 and the combined power for the 10 loci is 0.99989.
Keywords:
DNA typing, forensic science, Malay, Malaysia, population genetics
Paper ID: JFS2002373_482
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Author Panneerchelvam S, Haslindawaty N, Ravichandran M, Norazmi MN, Zainuddin ZF
Title Allele frequency distribution for 10 STR loci in the Malay population of Malaysia
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