A Spanish population study of the STR loci D2S1338, D19S433, Penta D, and Penta E
Fernández, L
Instituto Nacional de Toxicología , Seccón de Biología, Luis Cabrera 9, E-28002 Madrid, Spain.

García, O
Area de Laboratorio Ertzaintza, Avda. Montevido 3, E-48002 Bilbao, Spain.

Martín, P
Instituto Nacional de Toxicología , Seccón de Biología, Luis Cabrera 9, E-28002 Madrid, Spain.

Carcía, P
Instituto Nacional de Toxicología , Seccón de Biología, Luis Cabrera 9, E-28002 Madrid, Spain.

Sancho, M
Instituto Nacional de Toxicología , Seccón de Biología, Luis Cabrera 9, E-28002 Madrid, Spain.

Alonso, A
Instituto Nacional de Toxicología , Seccón de Biología, Luis Cabrera 9, E-28002 Madrid, Spain.


Abstract
The allele frequencies for the loci D2S1338, D195433, Penta D, and Penta E were determined in a Spanish population. Whole blood samples were obtained from 187-208 unrelated donors from Central Spain. Genomic DNA was extracted by a standard phenol/chloroform extraction procedure. PCR amplification was performed using the AmpFℓSTR SGM Plus D2S1338 and D19S433 loci—amplification kit (Applied Biosystems, Foster City, CA) and PowerPlex 16 Penta D and Penta E—amplification kit (Promega Corporation, Madison, WI) following manufacturer's instructions. The amplified products were separated and detected using the ABI 377 DNA sequencer (Applied Biosystems, Foster City, CA). Alleles were classified according to the recommendations of the ISFG (1). Statistical analysis was performed as previously reported (2). The complete dataset is available to any interested researcher upon request from the corresponding author

Keywords:
Central Spain, DNA typing, forensic science, population genetics, short tandem repeats

Paper ID: JFS2003064_485

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Author Fernández L, García O, Martín P, Carcía P, Sancho M, Alonso A Title A Spanish population study of the STR loci D2S1338, D19S433, Penta D, and Penta E Symposium , Committee on