Concordance study on population database samples using the PowerPlex™ 16 kit and AmpFLSTR® Profiler Plus™ kit and AmpFLSTR® COfiler™ kit
Budowle, B
Senior scientist, Laboratory Division, FBI Academy, Quantico, VA.

Sprecher, CJ
Senior scientist, Promega Corporation, 2800 Woods Hollow, Madison, WI.


Abstract
Over 500 population database samples comprising African Americans, Bahamians, and Southwestern Hispanics were typed using the PowerPlex™ 16 and the Profiler Plus™ COfiler™ kits. There was only one sample in which a typing difference was observed. An FGA heterozygote profile was observed using the PowerPlex™ 16 primers, and a single allele FGA profile was observed using Profiler Plus primers. Thus, the extant data suggest that the primers used in the PowerPlex™ 16, Profiler Plus™, and COfiler™ kits are reliable for typing reference samples destined for use in CODIS. In addition, African American, Bahamian, and Southwestern Hispanic databases have been established for the STR loci Penta D and Penta E. Both loci are highly polymorphic. The application of the product rule is valid for estimating the rarity of a multiple loci profile consisting of these two and the 13 core STR loci.

Keywords:
allele drop-out, AmpFlSTR® Profiler Plus™ Kit, AmpFlSTR®Cofiler Kit, CODIS, concordance, forensic science, GenePrint® PowerPlex™ 2.1 kit, Hardy-Weinberg expectations, Penta D, Penta E, PowerPlex™ 16 Kit, primers, stochastic effects, STR

Paper ID: JFS4630637

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Author Budowle B, Sprecher CJ Title Concordance study on population database samples using the PowerPlex™ 16 kit and AmpFLSTR® Profiler Plus™ kit and AmpFLSTR® COfiler™ kit Symposium , Committee on