Determination of lysergic acid diethylamide in body fluids by high-performance liquid chromatography and fluorescence detection--a more sensitive method suitable for routine use
Bergemann, D
Institut fur Rechtsmedizin der Universitat Munchen, Munchen, Germany.

Geier, A
Institut fur Rechtsmedizin der Universitat Munchen, Munchen, Germany.

von Meyer, L
Institut fur Rechtsmedizin der Universitat Munchen, Munchen, Germany.


Abstract
A new method for determination of lysergic acid diethylamide (LSD) in body fluids by high-performance liquid chromatography and fluorescence detection was developed based on previously published methods. The new method is suitable for confirmation of samples tested positive by immunoassay, avoiding loss of LSD by absorption to surfaces. The reduced loss of LSD results in improved sensitivity. This is achieved by adding ethylene glycol to the samples, which cover glass surfaces. This principle can similarly be used to improve analysis of other drugs.
Body fluids for analysis included urine and whole blood. An internal standard was applied for quantification of LSD.
The new method offers satisfying precision data and has a detection limit of less than 0.05 ng/L.

Keywords:
diethylamide, ethylene glycol, fluorescence detection, forensic science, forensic toxicology, high-performance liquid chromotography, lysergic acid

Paper ID: CH19372X

ASTM International is a member of CrossRef.
Author Bergemann D, Geier A, von Meyer L Title Determination of lysergic acid diethylamide in body fluids by high-performance liquid chromatography and fluorescence detection--a more sensitive method suitable for routine use Symposium , Committee on