Potential Forensic Applications of Minisatellite Variant Repeat (MVR) Mapping Using the Polymerase Chain Reaction (PCR) at D1S8
Yamamoto, T
Assistant Professors, Research Student, Assistant Professor and Professor, respectively, Departmentof Legal Medicine, Nagoya University School of Medicine, Nagoya, Japan.

Tamaki, K
Assistant Professors, Research Student, Assistant Professor and Professor, respectively, Departmentof Legal Medicine, Nagoya University School of Medicine, Nagoya, Japan.

Kojima, T
Assistant Professors, Research Student, Assistant Professor and Professor, respectively, Departmentof Legal Medicine, Nagoya University School of Medicine, Nagoya, Japan.

Uchihi, R
Assistant Professors, Research Student, Assistant Professor and Professor, respectively, Departmentof Legal Medicine, Nagoya University School of Medicine, Nagoya, Japan.

Katsumata, Y
Assistant Professors, Research Student, Assistant Professor and Professor, respectively, Departmentof Legal Medicine, Nagoya University School of Medicine, Nagoya, Japan.


Abstract
Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) at D1S8 (MS32) was applied to samples from various human tissues. All DNA samples obtained from an individual's organs at autopsy consistently gave the same digital diploid codes. Even 1 ng of genomic DNA was sufficient to obtain authentic diploid MVR coding ladders. MVR-PCR could be reliably applied to DNA isolated from bloodstains, saliva stains, seminal stains and plucked hair roots, and should become a powerful tool for individual identification in forensic investigations.

Keywords:
digital DNA typing, forensic science, human identification, individual identification, minisatellite, minisatellite variant repeat mapping, pathology and biology, polymerase chain reaction, polymerase chain reaction (PCR)

Paper ID: JFS393940743

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Author Yamamoto T, Tamaki K, Kojima T, Uchihi R, Katsumata Y Title Potential Forensic Applications of Minisatellite Variant Repeat (MVR) Mapping Using the Polymerase Chain Reaction (PCR) at D1S8 Symposium , Committee on