STP1506

    Performance of an Enzyme Electrode Designed for a Sulfide Monitoring Biosensor

    Published: Jan 2009


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    Abstract

    The construction and evaluation of an enzyme electrode designed for a sulfide monitoring biosensor, are reported in this work. The enzyme, which is immobilized in a carbon (graphite) paste with 1,1 ′ dimeth-ylferrocene, catalyzes the oxidation of sulfide to sulfur.Amperometric measurements were carried out at room temperature, and a fixed potential of 0.3 V with reference to a saturated calomel electrode, using Tris-HCI buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration range of 0–4mM, giving an average apparent Michaelis constant of 3mM, and maximum current responses between 0.7 and 3.6 uA. The sensitivity of the electrodes tested ranged from 0.2–0.3 μA mM−1.

    Keywords:

    Sulfide monitoring, electrochemical biosensor, enzyme electrode, sulfide oxidase


    Author Information:

    Sooknah, Reeta
    Natural Resources Canada, Ottawa, Ontario

    Papavinasam, Sankara
    Natural Resources Canada, Ottawa, Ontario

    Attard, Michael
    Natural Resources Canada, Ottawa, Ontario

    Revie, R. Winston
    Natural Resources Canada, Ottawa, Ontario

    Gould, W. Douglas
    Natural Resources Canada, Ottawa, Ontario

    Dinardo, Orlando
    Natural Resources Canada, Ottawa, Ontario


    Paper ID: STP48749S

    Committee/Subcommittee: G01.11

    DOI: 10.1520/STP48749S


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