SYMPOSIA PAPER Published: 01 January 1962
STP43682S

A Technique for Removing Plastic Backing Films in the Preparation of Electron Microscope Specimens

Source

A relatively simple procedure has been developed which eliminates many of the difficulties frequently encountered in dissolving the thick layers of soluble plastics used as primary replicas in two-stage replica processes and as reinforcing or “backing” layers in the preparation of thin support membranes and direct replicas. The apparatus used in this procedure is a standard Soxhlet extractor which is equipped with a Z-shaped specimen carrier made of fine wire screen. The specimen carrier fits inside the extractor tube where it is held at a level slightly higher than the siphon tube by a wire or glass stand. The general features of this apparatus are illustrated in Fig. 1.

Author Information

Beals, T., F.
School of Public Health, University of Michigan, Ann Arbor, Mich
Bigelow, W., C.
University of Michigan, Ann Arbor, Mich
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Details
Developed by Committee: E04
Pages: 155–159
DOI: 10.1520/STP43682S
ISBN-EB: 978-0-8031-5971-6
ISBN-13: 978-0-8031-6125-2