A Technique for Removing Plastic Backing Films in the Preparation of Electron Microscope Specimens

    Published: Jan 1962

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    A relatively simple procedure has been developed which eliminates many of the difficulties frequently encountered in dissolving the thick layers of soluble plastics used as primary replicas in two-stage replica processes and as reinforcing or “backing” layers in the preparation of thin support membranes and direct replicas. The apparatus used in this procedure is a standard Soxhlet extractor which is equipped with a Z-shaped specimen carrier made of fine wire screen. The specimen carrier fits inside the extractor tube where it is held at a level slightly higher than the siphon tube by a wire or glass stand. The general features of this apparatus are illustrated in Fig. 1.

    Author Information:

    Beals, T. F.
    Research Assistant, School of Public Health, University of Michigan, Ann Arbor, Mich

    Bigelow, W. C.
    Associate Professor, University of Michigan, Ann Arbor, Mich

    Committee/Subcommittee: E04.01

    DOI: 10.1520/STP43682S

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