STP573

    Analysis of Organochlorine Residues in Fish

    Published: Jan 1975


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    Abstract

    The analyses of fish for organochlorine residues (OC pesticides, PCBs, PCTs) present some special cleanup problems due mainly to the difficulty of removing coextracted oil. The oil causes erratic elution patterns of the residues from many chromatographic column adsorbents.

    Our overall approach to the extraction, cleanup, pre-GLC separations and GLC analysis, as well as the quantitation of PCBs are described. The use of deactivated Florisil in our cleanup procedure solved many of the earlier problems, and has given consistently excellent cleanup (less than 0.01 g remains from 0.5 g fat) and recovery (above 90 percent) of residues from fish and other biological tissues. The Florisil adsorption column is designed to tolerate up to 0.5 g of oil or fat, but with the initial Soxhlet extraction, an appropriate aliquot can be subjected to the column cleanup. An additional cleanup step involving Florisil column partitioning is described for coping with much larger fat samples, especially where very low residue levels are to be determined.

    Our approach to PCB quantitation and the use of reference standards are discussed. The proposed use of a 1:1 mixture of Aroclor 1254 and 1260 as reference for most biological samples is preferred.

    Keywords:

    water quality, fishes, pesticides, chlorine organic compounds, environmental tests, gas chromatography


    Author Information:

    Reynolds, LM
    Senior research scientist and technologist, Ontario Research Foundation, Sheridan Park, Ontario

    Cooper, T
    Senior research scientist and technologist, Ontario Research Foundation, Sheridan Park, Ontario


    Paper ID: STP39010S

    Committee/Subcommittee: D19.95

    DOI: 10.1520/STP39010S


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