Digital Library / STP / STP1482-EB / STP37697S

Development of an In Situ Biodegradation Technology by Using Anaerobic Micro-Organisms for Sediment Contaminated with Dioxins
Hata, T
Researcher, Research Center for Water Environment Technology, The University of Tokyo,

Kurisu, F
Assistant Professor, Research Center for Water Environment Technology, The University of Tokyo,

Yagi, O
Professor, Research Center for Water Environment Technology, The University of Tokyo,

Mori, H
Researcher, Materials and Geotechnical Engineering Research Group, Public Works Research Institute,

Kuwano, R
Senior Researcher, Materials and Geotechnical Engineering Research Group, Public Works Research Institute,

Kohashi, H
Head of Division, Material and Geotechnical Engineering Research Group, Public Works Research Institute,

Pages: 9    Published: Jan 2006

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Source: STP1482-EB

Abstract

In this paper, an in situ bioremediation method is proposed for dioxin contaminated sediments and its efficiency is assessed in laboratory tests including microbial analysis. The main outcomes of this research are (1) Proposal of an in situ bioremediation method using anaerobic micro-organisms monitored by molecular microbial techniques. (2) It was shown that the proposed method can reduce dioxin concentrations and can increase populations of the dehalogenating micro-organism (Dehalococcoides sp.). (3) It was further shown that the dioxin degradation processes can be monitored using polymerase chain reaction (PCR) denaturing gradient gel electrophoresis and real-time PCR. Microbial analysis using molecular techniques was useful for the monitoring and management of the sediment remediation process.

Keywords:
Contaminated sediments, Dioxins, Bioremediation, PCR, DGGE

Paper ID: STP37697S
Committee/Subcommittee: D18.14
DOI: 10.1520/STP37697S
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