STP766

    Evaluation of a Bacterial Bioluminescence Bioassay as a Method for Predicting Acute Toxicity of Organic Chemicals to Fish

    Published: Jan 1982


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    Abstract

    The relationship between the toxicity of 68 organic chemicals to fish and luminescent bacteria was evaluated. Chemicals for which 96-h median lethal concentration (LC50) values had been measured for fathead minnows (Pimephales promelas) in flow-through tests at the U.S. Environmental Protection Agency (EPA) Environmental Research Laboratory-Duluth, Minn., were tested using the Microtox toxicity analyzer (Beckman Instruments, Inc.). The Microtox system measures the decrease in luminescence of the bacterium Photobacterium phosphoreum in response to a toxicant. The toxicity end point is the 5-min median effective concentration (EC50), which is the concentration that causes a 50 percent reduction in light output.

    The correlation between fish and bacteria toxicity for a composite of industrial chemicals and pesticides had an R2 value of 0.65. However, the relationship of these two end points for a semihomologous series of industrial chemicals appeared to be firm, with R2 being 0.96 for common alcohols. The feasibility of using the bacterial bioassay as a screening test in a cost-effective testing scheme is discussed.

    Keywords:

    acute toxicity, median lethal concentration (LC, 50, ), median effective concentration (EC, 50, ), bioluminescence, bacteria, fathead minnow, organic chemical, aquatic toxicology, hazard assessment


    Author Information:

    Curtis, C
    Biologist and research chemist, U.S. Environmental Protection Agency, Environmental Research Laboratory-Duluth, Duluth, Minn.

    Lima, A
    Biologist and biostatistician, Center for Lake Superior Environmental Studies, University of Wisconsin-Superior, Superior, Wis.

    Lozano, SJ
    Biologist and biostatistician, Center for Lake Superior Environmental Studies, University of Wisconsin-Superior, Superior, Wis.

    Veith, GD
    Biologist and research chemist, U.S. Environmental Protection Agency, Environmental Research Laboratory-Duluth, Duluth, Minn.


    Paper ID: STP36717S

    Committee/Subcommittee: E35.14

    DOI: 10.1520/STP36717S


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