SEDL / STP / STP752-EB / STP35153S

Analytical Techniques for Fluorescent Chemicals Used as Systemic or External Wildlife Markers

Johns, BE
Physiologist and research chemist, Denver Wildlife Research Center, U.S. Fish and Wildlife Service, Denver, Colo.

Pan, HP
Physiologist and research chemist, Denver Wildlife Research Center, U.S. Fish and Wildlife Service, Denver, Colo.

Pages: 8    Published: Jan 1981

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Abstract

New approaches to wildlife marking with fluorescent chemicals were examined using the analytical techniques of fluorometry, chromatography, and tissue examination under ultraviolet light. A miniaturized, portable, and economical chromatography system was utilized. Quinacrine dihydrochloride was used as a marker and was found to be detectable at 50 ppb in 1 ml of blood. Rhodamine B was demonstrated to be a long-lasting marker of coyote (Canis latrans) claws and hair when the chemical was ingested orally and systemically distributed. The use of the analytical methods presented, in combination with fluorescent chemicals, will provide researchers and managers with additional ways to mark wildlife. The possibility of the systemic marking of hair with a variety of chemicals offers an expanded future for wildlife marking.