STP690

    Estimating Aufwuchs Biomass

    Published: Jan 1979


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    Abstract

    Estimates of Aufwuchs biomass derived from gravimetric, phytopigment, and adenosine triphosphate (ATP) analyses are evaluated and compared with respect to each method's specificities and biases. Samples of Aufwuchs from glass slides in artificial streams treated with chlorine, copper, dichromate, sucrose, dextrose, phosphate, or nitrogen were used to compare these nontaxonomic methods as water quality assessment techniques. The correlations and limitations of the methods are discussed. ATP analysis compared very favorably with the other techniques and added another important dimension in water quality assessment. When corrected for extraction efficiency, ATP analyses provide a reliable estimate of Aufwuchs biomass. This estimate can be used in combination with other estimates of biomass to partition the community into autotrophic-heterotrophic components or viable organic carbon and non- viable organic carbon. Ratios of organic carbon estimates such as chlorophyll a organic carbon/-ash-free dry weight organic carbon (Trophic Index), chlorophyll a organic carbon/ATP organic carbon (Functional Trophic Index), and ATP organic carbon/- ash-free dry weight organic carbon (Viability Index) were used to further assess the impact of perturbations on the biomass of the Aufwuchs community.

    Keywords:

    Aufwuchs, biomass, water quality assessment, dry weight, ash free dry weight, chlorophyll, adenosine triphosphate (ATP), organic carbon, periphyton, micro-communities, measurements


    Author Information:

    Clark, JR
    Graduate research assistant, associate professor and assistant director, and university distinguished professor, Center for Environmental Studies and Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Va.

    Dickson, KL
    Graduate research assistant, associate professor and assistant director, and university distinguished professor, Center for Environmental Studies and Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Va.

    Cairns, J
    Graduate research assistant, associate professor and assistant director, and university distinguished professor, Center for Environmental Studies and Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Va.


    Paper ID: STP35067S

    Committee/Subcommittee: D19.24

    DOI: 10.1520/STP35067S


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