STP737

    On-Site Critical Life-Stage Bioassay with the Fathead Minnow, Pimephales promelas, on Effluent from the Wastewater Treatment Facilities of Austin, Minn.

    Published: Jan 1981


      Format Pages Price  
    PDF Version (172K) 12 $25   ADD TO CART
    Complete Source PDF (6.2M) 12 $55   ADD TO CART


    Abstract

    An on-site, flow-through, critical life-stage bioassay was conducted challenging the fathead minnow with treated effluent from the City of Austin, Minn., wastewater treatment facilities. Using U.S. Environmental Protection Agency (EPA) methodology modified by Union Carbide Environmental Services (UCES) and the Minnesota Pollution Control Agency (MPCA), effluent effect data were obtained for (a) egg hatchability, (b) fry survival, (c) fry growth, and (d) fry deformities. The data were used to estimate the maximum acceptable effluent concentration (MAEC) range, expressed as percent effluent and ammonia concentration. Statistical analyses (P = 0.05) indicated an MAEC range of 18 to 32 percent treated effluent. The corresponding un-ionized and total ammonia concentrations were, respectively, 0.2 to 0.3 mg/litre and 2.3 to 3.2 mg/litre.

    In light of the current EPA water quality criterion of 0.02 mg/litre un-ionized ammonia, the results suggest that in situ studies of this type might be useful in establishing more realistic discharge requirements.

    Keywords:

    ammonia, bioassay, effluent, fathead minnow, fish, toxicity, aquatic toxicology, hazard assessment


    Author Information:

    Iwan, GR
    Section leader, Quality Assurance, and Section leader, Field Studies, Union Carbide Environmental Services, Union Carbide Corp., Tarrytown, N.Y.

    Cella, GE
    Section leader, Quality Assurance, and Section leader, Field Studies, Union Carbide Environmental Services, Union Carbide Corp., Tarrytown, N.Y.


    Paper ID: STP34164S

    Committee/Subcommittee: D19.15

    DOI: 10.1520/STP34164S


    CrossRef ASTM International is a member of CrossRef.