STP737: Metabolites of Benzo[a]pyrene in Aroclor 1254-Treated Mullet

    Tan, B
    Research associate, graduate students, and professor of chemistry, Auburn University, Auburn, Ala.

    Kilgore, MV
    Research associate, graduate students, and professor of chemistry, Auburn University, Auburn, Ala.

    Elam, DL
    Research associate, graduate students, and professor of chemistry, Auburn University, Auburn, Ala.

    Melius, P
    Research associate, graduate students, and professor of chemistry, Auburn University, Auburn, Ala.

    Schoor, WP
    Staff member, U.S. Environmental Protection Agency, Environmental Research Laboratory, Sabin Island, Gulf Breeze, Fla.

    Pages: 8    Published: Jan 1981


    Abstract

    This paper presents a systematic approach to the separation and characterization of benzo[a]pyrene (BaP) metabolites using the efficient analytical technique of high-pressure liquid chromatography (HPLC). Hepatic supernatant (S-9) preparations from Aroclor 1254-treated mullet (Mugil cephalus) were used to study the in vitro metabolism of BaP. The lipid-soluble hydroxylated products were extracted in ethyl acetate prior to HPLC analysis. Three diols (9,10; 7,8; and 4,5) and two phenols (9 and 3) of BaP were isolated and identified. From their relative retention times, the other metabolites noted were likely to be unresolvable quinones. The results obtained in the mullet were compared with those obtained in the rat (Rattus norvegicus).

    Keywords:

    polynuclear aromatic hydrocarbon, benzo[, a, ]pyrene, benzo[, a, ]pyrene metabolites, high-pressure liquid chromatography, marine organisms, Mugil cephalus, Rattus norvegicus, Aroclor 1254, aquatic toxicology, hazard assessment


    Paper ID: STP34160S

    Committee/Subcommittee: D19.05

    DOI: 10.1520/STP34160S


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