STP971: A Short-Term Chronic Toxicity Test Using Daphnia Magna

    Lewis, PA
    Aquatic biologist and chief, Aquatic Biology Section, Biological Methods Branch, Environmental Monitoring and Support Laboratory—Cincinnati, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, OH

    Horning, WB
    Aquatic biologist and chief, Aquatic Biology Section, Biological Methods Branch, Environmental Monitoring and Support Laboratory—Cincinnati, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, OH

    Pages: 8    Published: Jan 1988


    Abstract

    A seven-day short-term chronic toxicity test using ten-day-old Daphnia magna is described as a possible alternative to the Ceriodaphnia short-term test. The test was begun on Friday so that weekend work was limited to feeding. The test was conducted at 25°C, and the animals were fed daily.

    Five tests using sodium pentachlorophenate conducted during the development of the method gave test results similar to those previously reported for Daphnia magna chronic tests and comparable to Ceriodaphnia short-term tests conducted at the U.S. Environmental Monitoring and Support Laboratory (EMSL), Cincinnati. This test can be completed with about half the labor required for the Ceriodaphnia test, and the neonates can be easily counted without the aid of a microscope.

    Keywords:

    toxicity, chronic test, Daphnia magna, Ceriodaphnia, sodium pentachlorophenate


    Paper ID: STP34067S

    Committee/Subcommittee: E47.01

    DOI: 10.1520/STP34067S


    CrossRef ASTM International is a member of CrossRef.