STP802

    Effect of Cadmium on Lake Water Bacteria as Determined by the Luciferase Assay of Adenosine Triphosphate

    Published: Jan 1983


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    Abstract

    A firefly luciferase assay of bacterial adenosine triphosphate (ATP) was developed to measure the toxic effects of cadmium ions on aquatic organisms. Toxicity was monitored using intracellular (I/C) ATP (in micrograms per litre) as well as plate counts (colony-forming units per millilitre). The bacteria, which belonged mainly to the families Enterobacteriaceae and Pseudomonadaceae, exhibited varying degrees of resistance to up to 100 ppm cadmium when grown in a glucose-salts medium at pH 6.8. Among the organisms tested, cadmium resistance decreased in the following order: Pseudomonas vesicularis > P. aeruginosa > Enterobacter sp. > P. fluorescens > Chromobacter sp. > Serratia sp. A rise in the pH of the growth medium from 5 to 7 resulted in increased toxicity of cadmium.

    Keywords:

    adenosine triphosphate (ATP), bioluminescence, cadmium, aquatic bacteria, plate counts, pH, aquatic toxicology, hazard assessment


    Author Information:

    Seyfried, PL
    Associate professor and graduate student, University of Toronto, Toronto, Ont.

    Horgan, CBL
    Associate professor and graduate student, University of Toronto, Toronto, Ont.


    Paper ID: STP33521S

    Committee/Subcommittee: E47.01

    DOI: 10.1520/STP33521S


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