SEDL / STP / STP898-EB / STP33292S

Biochemistry of Seeded Endothelial Cells

Sharp, WV
Director, Vascular Research Laboratory, Akron City Hospital, Akron, OH

Schmidt, SP
Associate directoradjunct associate professor of biology, Research Laboratory, Akron City HospitalInstitute for Biomedical Engineering Research, The University of Akron, AkronAkron, OHOH

Hunter, TJ
Research technician, Vascular Research Laboratory, Akron City Hospital, Akron, OH

Pages: 8    Published: Jan 1986

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Abstract

The beneficial effect of seeding endothelial cells on synthetic vascular conduits has been well established by Herring, Graham, Schmidt, and others. This report discusses the biochemical production and interaction of the prostaglandins, prostacyclin (PGI²) and thromboxane (TXA²). A series of animal implantations was carried out with seeded and nonseeded grafts. Animals were medicated with cyclooxygenase inhibitors and TXA² synthase inhibitors. Analysis was performed of PGI² and TXA² production on seeded and nonseeded grafts. Results of this analysis indicate: (1) seeded endothelial cells in Dacron® velour grafts can synthesize PGI², (2) the levels of PGI² from seeded endothelial cells are far less than the adjacent carotid artery, (3) the biochemistry of PGI² production can be modulated by various antiplatelet agents, and (4) TXA² synthase inhibitors improve thromboresistance of seeded grafts without significant effect on PGI² production.