SEDL / STP / STP846-EB / STP33228S

Optimizing Reproducibility in the Analysis of Polymers by Steric Exclusion Chromatography

Miller, R
Senior applications chemist and product specialist, Perkin-Elmer Corporation, Norwalk, CT

Dalton, G
Senior applications chemist and product specialist, Perkin-Elmer Corporation, Norwalk, CT

Pages: 15    Published: Jan 1984

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Abstract

Several potential sources of variation in steric exclusion chromatography (SEC) are discussed: flow rate stability of the pump, column temperature, data processing, and the chromatography itself. Many of these variation sources may be minimized by careful attention to analytical techniques. Flow-rate stability, however, is a function of the chromatographic hardware used and is not as readily subject to control. Experimental techniques are described whereby relative standard deviations below 0.3% in the routine determinations of molecular-weight averages may be achieved on an optimized chromatographic system.