SYMPOSIA PAPER Published: 01 January 1984
STP33228S

Optimizing Reproducibility in the Analysis of Polymers by Steric Exclusion Chromatography

Source

Several potential sources of variation in steric exclusion chromatography (SEC) are discussed: flow rate stability of the pump, column temperature, data processing, and the chromatography itself. Many of these variation sources may be minimized by careful attention to analytical techniques. Flow-rate stability, however, is a function of the chromatographic hardware used and is not as readily subject to control. Experimental techniques are described whereby relative standard deviations below 0.3% in the routine determinations of molecular-weight averages may be achieved on an optimized chromatographic system.

Author Information

Miller, R
Perkin-Elmer Corporation, Norwalk, CT
Dalton, G
Perkin-Elmer Corporation, Norwalk, CT
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Details
Developed by Committee: E11
Pages: 51–65
DOI: 10.1520/STP33228S
ISBN-EB: 978-0-8031-4912-0
ISBN-13: 978-0-8031-0408-2