STP634

    Uptake, Clearance, and Bioconcentration of 14C-Sec-Butyl-4-Chlorodiphenyl Oxide in Rainbow Trout

    Published: Jan 1977


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    Abstract

    Steady state bioconcentration factors for sec-butyl-4-chlorodiphenyl oxide in rainbow trout muscle were calculated using a simple two-compartment model from radioassay data of concentrations of 14C in fish and water during an uptake and clearance (depuration) experiment. There was a relatively rapid removal with one half of the material cleared in 30 to 40 h.

    Average Exposure Concentration, ppbUptake Rate, k1, ppb/ppb/hClearance Rate, k2, h−1Bioconcentration FactorClearance Half Time, hTime to 90% of Steady State, h3.626.1 ± 1.00.021 ± 0.004292 ± 7533 ± 7111 ± 220.345.3 ± 0.50.016 ± 0.002336 ± 5244 ± 5146 ± 18

    A partition coefficient (octanol/water) for sec-butyl-4-chlorodiphenyl oxide of 16000 ± 4900 was measured. The regression equation relating the partition coefficient to bioconcentration factor in trout muscle predicts a bioconcentration factor of 100 to 500, in agreement with the calculated values of 292 and 336.

    The two-compartment model used for analysis of the data does not distinguish between 14C activity in the form of sec-butyl-4-chlorodiphenyl oxide from that in the form of metabolic products. The reported bioconcentration factor is an overall number encompassing both the compound and its metabolites. Thin-layer chromatography analysis of tissue extracts showed polar metabolites were formed during the experiment. The calculated bioconcentration factors are somewhat larger than they would be if put on a pure compound basis. However, we believe that the reported bioconcentration factors are valid, since there is good agreement between the 14C-derived muscle bioconcentration factors and the bioconcentration factor derived from the partition coefficient equation.

    Keywords:

    toxicology, water analysis, uptake rate constant, clearance rate constant, bioconcentration, 14, C-, sec, -butyl-4-chlorodiphenyl oxide, trout, partition coefficient, capacitor fluid, metabolites, kinetic model


    Author Information:

    Blanchard, FA
    Senior research specialists, research specialist, and chemist, Environmental Sciences Research, The Dow Chemical Company, Midland, Mich.

    Takahashi, IT
    Senior research specialists, research specialist, and chemist, Environmental Sciences Research, The Dow Chemical Company, Midland, Mich.

    Alexander, HC
    Senior research specialists, research specialist, and chemist, Environmental Sciences Research, The Dow Chemical Company, Midland, Mich.

    Bartlett, EA
    Senior research specialists, research specialist, and chemist, Environmental Sciences Research, The Dow Chemical Company, Midland, Mich.


    Paper ID: STP32398S

    Committee/Subcommittee: E35.13

    DOI: 10.1520/STP32398S


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