STP822

    Investigations on the Use of Laser Dyes as Quantum Counters for Obtaining Corrected Fluorescence Spectra in the Near Infrared

    Published: Jan 1983


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    Abstract

    Several laser dyes were evaluated as quantum counters for obtaining corrected fluorescence spectra in the near infrared regions. Preliminary results indicate that hexamethylindotricarbocyanine (HITC) and LDS-798 (Exciton Chemical Co., Overlook, OH) both have quantum yields that are independent of wavelength and can extend the limits of spectral correction to 800 and 700 nm, respectively. A binary quantum counter containing a mixture of Rhodamine B (RhB) and HITC was also evaluated. The quantum yields of HITC and LDS-798 were estimated to be 0.28 and 0.14, respectively, compared to a value of 0.95 attributed to RhB. Examples of corrected spectra obtained using LDS-798 and HITC are compared with absorbance spectra and other accepted standards. Preliminary stability tests are discussed and further studies suggested.

    Keywords:

    fluorescence spectrophotometers, corrected spectra, quantum counters, laser dyes, molecular luminescence


    Author Information:

    Duggan, JX
    Product specialist, fluorescence, Perkin-Elmer Corp., Oak Brook Instrument Division, Oak Brook, IL

    DiCesare, J
    Product manager, liquid chromatography and senior product specialist, infrared, Perkin-Elmer Corp., Norwalk, CT

    Williams, JF
    Product manager, liquid chromatography and senior product specialist, infrared, Perkin-Elmer Corp., Norwalk, CT


    Paper ID: STP31871S

    Committee/Subcommittee: E13.06

    DOI: 10.1520/STP31871S


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