SYMPOSIA PAPER Published: 01 January 1983
STP31868S

Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry

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The technique of multifrequency phase and modulation fluorometry may be applied to the measurement and analysis of heterogeneous fluorescence lifetimes. This paper addresses two approaches to the data analysis: (1) the exact analytical solution (Weber's algorithm) for determining the lifetimes of N components and their relative contributions using N modulation frequencies and (2) a least-squares method. The mathematical rationale for each approach is described and the effects of random or systematic errors on the results obtained with each method are discussed. The relative merits of each approach are considered in light of results on both real and simulated data.

Author Information

Jameson, DM
University of Texas, Health Science Center at Dallas, Dallas, TX
Gratton, E
University of Illinois at Urbana-Champaign, Urbana, IL
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Details
Developed by Committee: E13
Pages: 67–81
DOI: 10.1520/STP31868S
ISBN-EB: 978-0-8031-4887-1
ISBN-13: 978-0-8031-0212-5