SEDL / STP / STP810-EB / STP30157S

Assessment of Biocompatibility of Orofacial Materials and Devices by Culturing with Human Excised Donor Tissues

Lontz, JF
Associate director, research biologist, and biochemist, Maxillofacial Prosthetics Center, Veterans Administration Medical Center, Wilmington, Del.

Nadijcka, MD
Associate director, research biologist, and biochemist, Maxillofacial Prosthetics Center, Veterans Administration Medical Center, Wilmington, Del.

Holmes, R
Associate director, research biologist, and biochemist, Maxillofacial Prosthetics Center, Veterans Administration Medical Center, Wilmington, Del.

Pages: 11    Published: Jan 1983

Download this paper for $25 PDF (244K)          View License Agreement
Abstract

Three methods for assessing biocompatibility of materials used in orofacial constructs have been studied. These methods are (1) human excised donor (HED) orofacial tissues cultured in medium containing processed human serum, (2) an established cell line cultured in a serum free medium, and (3) an established human cell line cultured in a medium containing processed human serum. Of the three methods, the first was preferred since the cells used were removed recently from their total human physiology and may provide the most realistic evaluation of human-tissue response.