STP921

    Passive Perchorionic Carcinogen Bioassay Using Rainbow Trout (Salmo gairdneri) Embryos

    Published: Jan 1986


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    Abstract

    A noninvasive method for exposing eyed-stage rainbow trout (Salmo gairdneri) embryos to chemicals in a bioassay for toxicity and carcinogenicity was tested with four known mammalian carcinogens. Aflatoxin B1 (AFB1), benzo[a]pyrene (BaP), dimethylnitrosamine (DMN), and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were applied topically in dimethyl sulfoxide (DMSO) to the chorion of developing rainbow trout embryos. All chemicals were toxic and decreased viability of fry after hatching. In addition, AFB1 (90 ng/egg) increased mortality in the egg stage and was the most toxic chemical tested. After a latent period of eight months, all chemicals had induced liver neoplasms in surviving fingerlings. The incidence of liver neoplasia ranged from 4.8% for DMN at 170.7 µg/egg to 76.9% for MNNG at 20.5 µg/egg and generally was in the form of basophilic foci of altered hepatocytes. Eleven months post exposure, all treatment groups had individuals with well-differentiated hepatocellular carcinomas. The data suggest that the method has the potential to be a useful bioassay for chemical carcinogenicity.

    Keywords:

    rainbow trout (, Salmo gairdneri, ), in vivo, bioassay, chemical carcinogens, topical application, dimethyl sulfoxide (DMSO), aquatic toxicology, hazard assessment


    Author Information:

    Maccubbin, AE
    Cancer research scientist II and cancer research scientist IV, Roswell Park Memorial Institute, Buffalo,

    Black, JJ
    Cancer research scientist II and cancer research scientist IV, Roswell Park Memorial Institute, Buffalo,


    Paper ID: STP29032S

    Committee/Subcommittee: E47.10

    DOI: 10.1520/STP29032S


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