STP921

    Biotransformation of Benzo(a)pyrene by Mercenaria mercenaria and Crassostrea virginica

    Published: Jan 1986


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    Abstract

    Using a sensitive, radioisotopic assay for aryl hydrocarbon hydroxylase (AHH), a comparatively low level of benzo(a)pyrene (BaP) metabolism was routinely measured in digestive gland homogenates. Attempts to induce overall BaP metabolism by exposure to Aroclor 1254, an inducer of mammalian AHH, were largely unsuccessful. However, Aroclor treatment produced an altered BaP metabolite profile for each bivalve species tested. The metabolites were separated and identified by coelution with authentic standards, using high-performance liquid chromatography. Untreated mollusks produced several dihydrodiols and phenolic derivatives; Aroclor treatment usually resulted in augmented generation of 9, 10-, 4,5-, and 7,8-diols, as well as production of quinones and atypical monohydroxylated metabolites. The data suggest that BaP biotransformation by bivalve enzyme systems can yield both potentially carcinogenic (BaP 7,8-diol) and detoxified metabolites. However, no apparent activation of BaP by molluscan enzymes was seen using the Ames Salmonella tester strains.

    Keywords:

    environmental carcinogenesis, biotransformation, benzo(a)pyrene, carcinogen metabolism, Mercenaria mercenaria, clams, Crassostrea virginica, oysters, mixed function oxygenases, aryl hydrocarbon hydroxylase, cytochrome P-450, aquatic toxicology


    Author Information:

    Anderson, RS
    Laboratory head and research assistant, Sloan-Kettering Institute for Cancer Research, Rye, NY

    Chesapeake Biological Laboratory, University of Maryland, Solomons, MD

    Angel, MA
    Laboratory head and research assistant, Sloan-Kettering Institute for Cancer Research, Rye, NY

    Chesapeake Biological Laboratory, University of Maryland, Solomons, MD


    Paper ID: STP29029S

    Committee/Subcommittee: E47.04

    DOI: 10.1520/STP29029S


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