Associate aquatic toxicologist, Illinois Natural History Survey, Champaign, Il
Agent de recherche, Université de Montréal, Montréal, Québec
Survey scientist, Inland Waters Directorate, Environment Canada, Longueuil, Québec
Pages: 6 Published: Jan 1988
A toxicity bioassay using the green alga Selenastrum capricornutum and measuring inhibition of photosynthetic carbon-14 (14C)-labeled carbon dioxide (14CO2) assimilation is described. The essential test specifications are the following:
(a) experimental vessels: 16-mL test tubes;
(b) exposure and incubation times: 20 h exposure plus 4 h incubation;
(c) dilution water: demineralized H2O;
(d) algal cell density: 1.9 × 105 cells mL−1;
(e) radioactivity: 0.072 µCi mL−1, as 14C-labeled sodium bicarbonate [Na2(H14CO3)2];
(f) nutrient enrichment: 1 mL of × 10 provisional algal assay procedure (PAAP) medium; and
(g) isotope partitioning: acidification and bubbling.
The resulting method is rapid, sensitive, and reliable (coefficient of variation < 10%) and is applied to elutriates of St. Lawrence River sediments in an ongoing research project.
hazard evaluation, toxicity, bioassays, algae, Selenastrum capricornutum, sediments, elutriates, carbon-14-labeled carbon dioxide (, 14, CO, 2, ), 14, CO, 2, uptake, St. Lawrence River, copper chloride
Paper ID: STP26256S