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A Rapid Bioassay Using the Green Alga Selenastrum capricornutum to Screen for Toxicity in St. Lawrence River Sediment Elutriates Pages: 6 Published: Jan 1988
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View License Agreement Source: STP988-EB Abstract A toxicity bioassay using the green alga Selenastrum capricornutum and measuring inhibition of photosynthetic carbon-14 (14C)-labeled carbon dioxide (14CO2) assimilation is described. The essential test specifications are the following: (a) experimental vessels: 16-mL test tubes; (b) exposure and incubation times: 20 h exposure plus 4 h incubation; (c) dilution water: demineralized H2O; (d) algal cell density: 1.9 × 105 cells mL−1; (e) radioactivity: 0.072 µCi mL−1, as 14C-labeled sodium bicarbonate [Na2(H14CO3)2]; (f) nutrient enrichment: 1 mL of × 10 provisional algal assay procedure (PAAP) medium; and (g) isotope partitioning: acidification and bubbling. The resulting method is rapid, sensitive, and reliable (coefficient of variation < 10%) and is applied to elutriates of St. Lawrence River sediments in an ongoing research project. Keywords: hazard evaluation, toxicity, bioassays, algae, Selenastrum capricornutum, sediments, elutriates, carbon-14-labeled carbon dioxide (, 14, CO, 2, ), 14, CO, 2, uptake, St. Lawrence River, copper chloride Paper ID: STP26256S Committee/Subcommittee: D19.24 DOI: 10.1520/STP26256S ASTM International is a member of CrossRef. | ||