STP1096

    Reduction in the Numbers of Antibody-Producing Cells in Rainbow Trout, Oncorhynchus mykiss, Exposed to Sublethal Doses of Phenol Before Bath Immunization

    Published: Jan 1990


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    Abstract

    The numbers of splenic antibody-producing cells were reduced in groups of fish given a 2, 5, or 30 min bath exposure to 10 ppm phenol before immunization by bath with the bacterin, Yersinia ruckeri O-antigen. The lethal time of 50% (LT50) for the 10 ppm phenol bath was 45 min. The passive hemolytic plaque assay was used to monitor the numbers of antibody-producing cells in the phenol-pretreated and sham-treated fish 14 or 21 days after contaminant exposure and 5 min bath immunizations at 10 or 100 μg/mL. These results, documenting a suppression of the immune response to bath immunization following a brief exposure to a toxicant, may help to explain delayed mortalities where pollution is implicated.

    Keywords:

    rainbow trout, immunosuppression, pollution, phenol, gill cells, bath immunization, fisheries, antibody-producing cells, sublethal effects


    Author Information:

    Anderson, DP
    U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Kearneysville, WV

    Dixon, OW
    U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Kearneysville, WV

    van Muiswinkel, WB
    Agricultural University, Zodiac, Wageningen,


    Paper ID: STP20116S

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP20116S


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