Research biologist, U.S. Environmental Protection Agency, Research Triangle Park, NC
North Carolina Central University, Durham, NC
Project leader, Environmental Health Research and Testing, Inc., Research Triangle Park, NC
Pages: 5 Published: Jan 1991
A short-term assay using Chinese Spring wheat for detecting induced aneuploidy and/or clastogenicity is described. Seeds of virescent wheat (v1v1) are treated with a test agent. The appearance of white sectors (v1v1v1) or green sectors (v10) on the virescent leaves indicates the gain or loss of a chromosome or a segment of a chromosome-bearing v1 allele. Three test chemicals, chromium trioxide (100 to 5000 (μg/mL), chromium dichromate (500 to 5000 μg/mL), and cyclophosphamide (100 to 1000 μg/mL) produced a dose-related positive response for chromosome aberrations in this assay.
wheat seedling assay, aneuploidy, clastogenicity, genotoxicity
Paper ID: STP19527S