STP1115

    Identification of Aneuploidy-Inducing Agents Using the Wheat Seedling Assay

    Published: Jan 1991


      Format Pages Price  
    PDF (92K) 5 $25   ADD TO CART
    Complete Source PDF (6.8M) 5 $104   ADD TO CART


    Abstract

    A short-term assay using Chinese Spring wheat for detecting induced aneuploidy and/or clastogenicity is described. Seeds of virescent wheat (v1v1) are treated with a test agent. The appearance of white sectors (v1v1v1) or green sectors (v10) on the virescent leaves indicates the gain or loss of a chromosome or a segment of a chromosome-bearing v1 allele. Three test chemicals, chromium trioxide (100 to 5000 (μg/mL), chromium dichromate (500 to 5000 μg/mL), and cyclophosphamide (100 to 1000 μg/mL) produced a dose-related positive response for chromosome aberrations in this assay.

    Keywords:

    wheat seedling assay, aneuploidy, clastogenicity, genotoxicity


    Author Information:

    Sandhu, SS
    Research biologist, U.S. Environmental Protection Agency, Research Triangle Park, NC

    Dhesi, JS
    North Carolina Central University, Durham, NC

    Gill, BS
    Project leader, Environmental Health Research and Testing, Inc., Research Triangle Park, NC


    Paper ID: STP19527S

    Committee/Subcommittee: E47.04

    DOI: 10.1520/STP19527S


    CrossRef ASTM International is a member of CrossRef.