SEDL / STP / STP1115-EB / STP19527S

Identification of Aneuploidy-Inducing Agents Using the Wheat Seedling Assay

Sandhu, SS
Research biologist, U.S. Environmental Protection Agency, Research Triangle Park, NC

Dhesi, JS
North Carolina Central University, Durham, NC

Gill, BS
Project leader, Environmental Health Research and Testing, Inc., Research Triangle Park, NC

Pages: 5    Published: Jan 1991

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Abstract

A short-term assay using Chinese Spring wheat for detecting induced aneuploidy and/or clastogenicity is described. Seeds of virescent wheat (v¹v¹) are treated with a test agent. The appearance of white sectors (v¹v¹v¹) or green sectors (v¹0) on the virescent leaves indicates the gain or loss of a chromosome or a segment of a chromosome-bearing v¹ allele. Three test chemicals, chromium trioxide (100 to 5000 (μg/mL), chromium dichromate (500 to 5000 μg/mL), and cyclophosphamide (100 to 1000 μg/mL) produced a dose-related positive response for chromosome aberrations in this assay.