STP1115

    Activation of Aflatoxin B1 and Benzo(a)pyrene by Tobacco Cells in the Plant Cell/Microbe Coincubation Assay

    Published: Jan 1991


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    Abstract

    We investigated the ability of tobacco (TX1) cells in culture to activate aflatoxin B1 (AFB1) and benzo(a)pyrene (BAP) using the plant cell/microbe coincubation assay. Initial experiments with Salmonella typhimurium as a genetic endpoint using previously published assay conditions failed to demonstrate that either of these agents could be activated into a mutagenic form by TX1 cells. However, when modifications of the assay, including increased plant cell densities and/or increased preincubation times, were employed, both AFB1 and BAP were demonstrated to be potent plant promutagens. These data demonstrate that, for the purpose of routine screening and toxicity assessment studies focusing upon plant activation, careful attention must be paid to both the chemical or complex mixture under study as well as to the metabolic potential of the particular plant cell system employed. This will ensure a more complete understanding of the problem at hand, provide insight into the mechanisms of activation, and guard against possible false negative results.

    Keywords:

    Salmonella, plant activation, aflatoxin B1, benzo(a)pyrene, plant cell/microbe coincubation assay


    Author Information:

    Gentile, JM
    Dean, Natural Sciences and Kenneth G. Herrick Professor of Biology, Hope College, MI

    Johnson, P
    Undergraduates, Hope College, Holland, MI

    Robbins, S
    Undergraduates, Hope College, Holland, MI


    Paper ID: STP19526S

    Committee/Subcommittee: E47.04

    DOI: 10.1520/STP19526S


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