SEDL / STP / STP1115-EB / STP19519S

Tissue Culture as a Method for Evaluating the Biotransformation of Xenobiotics by Plants

Wickliff, C
Biologist, U.S. Environmental Protection Agency, Environmental Research Laboratory, Corvallis, OR

Fletcher, JS
Professor of botany, University of Oklahoma, Norman, OK

Pages: 8    Published: Jan 1991

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Abstract

Suspension cultures of Rosa cultivar (cv.) Paul's Scarlet were used as a model system to examine the metabolism of 1,3-dinitrobenzene (DNB), an industrial waste compound. In a 3-day period, 90% of the DNB supplied (96 nmol) was metabolized by approximately 12 g (fresh weight) of cells. The primary end product of DNB metabolism partitioned into the insoluble residue fraction of the extracted cells. This study demonstrates the usefulness of plant tissue cultures in evaluating the chemical influence of plants on xenobiotics.