Characterization of Eight Soil Types Using the Selenastrum Capricornutum Bioassay

    Published: Jan 1991

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    A recently developed bioassay protocol was used to characterize seven different soil types and Promix BX. The green alga Selenastrum capricornutum was used as the test organism. A ruggedness test which altered seven variables was conducted to identify procedural stipulations that should be carefully controlled in the Selenastrum capricornutum bioassay. Stimulatory or inhibitory effects as measured by population growth were compared to a control using two different nutritional methods termed proportional amendment (PA) and complete amendment (CA). Population growth, calculated effect (CE) values, and EC50 values are given for each soil. A wide range of effects were documented indicating a substantial difference among soils with respect to their ability to stimulate or inhibit algal population growth.


    Selenastrum capricornutum, bioassay, proportional amendment, complete amendment, calculated effect, EC, 50, ruggedness test, soils

    Author Information:

    Ireland, FA
    Research animal scientist, Dixon Springs Agricultural Center, University of Illinois, Simpson, IL

    Judy, BM
    Research specialist, University of Missouri, Columbia, MO

    Lower, WR
    Group leader, Environmental Trace Substances Research Center, University of Missouri, Columbia, MO

    Thomas, MW
    Vegetation specialist, American Cyanamid Company, Birmingham, AL

    Krause, GF
    Professor and instructor, University of Missouri, Columbia, MO

    Asfaw, A
    Professor and instructor, University of Missouri, Columbia, MO

    Sutton, WW
    Senior biologist, Environmental Research Laboratory, U.S. Environmental Protection Agency, Athens, GA

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP19516S

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