SEDL / STP / STP1062-EB / STP18963S

Evaluation and Validation of Protocols for Measurement of the Mutagenicity of Environmental Samples

Wang, YY
Environmental BiochemistsStaff ScientistChiefActing Director, Air and Industrial Hygiene LaboratoryS-CUBEDEMSL-LV, EPAEMSL-LV, EPA, BerkeleyLa Jolla, CACA

Marsden, PJ
Environmental BiochemistsStaff ScientistChiefActing Director, Air and Industrial Hygiene LaboratoryS-CUBEDEMSL-LV, EPAEMSL-LV, EPA, BerkeleyLa Jolla, CACA

Flessel, CP
Environmental BiochemistsStaff ScientistChiefActing Director, Air and Industrial Hygiene LaboratoryS-CUBEDEMSL-LV, EPAEMSL-LV, EPA, BerkeleyLa Jolla, CACA

Williams, LR
Environmental BiochemistsStaff ScientistChiefActing Director, Air and Industrial Hygiene LaboratoryS-CUBEDEMSL-LV, EPAEMSL-LV, EPA, BerkeleyLa Jolla, CACA

Pages: 21    Published: Jan 1990

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Abstract

Protocols have been validated for the preparation of wastewater, drinking water, soil, and sediment samples for the Ames Salmonella mutagenicity assay. These protocols are proposed for use with environmental samples in “Guidelines for Preparing Environmental Waste Samples for Mutagenicity (Ames) Testing: Interim Procedures and Panel Meeting Proceedings, EPA 600/4-85-058 [1] and are validated essentially as written. Samples are extracted using EPA methods [2], concentrated, solvent exchanged, and assayed in strains TA98 and TA100 with and without S-9 activation. The performance of each method is optimized using environmental samples with variables of sample size and extraction solvent. Bias of the methods is established by using samples fortified with reference mutagens; precision is determined by testing replicate aliquots of a variety of samples. This work is an on-going effort sponsored by EMSL-LV to produce robust, reliable procedures for the measurement of mutagenicity in environmental media.