STP1102

    Detection of Human Enteroviruses Recovered from Water by the Streptavidin-Biotin/Nitrocellulose-Enzyme Immunoassay Procedure (SAB/NC-EIA)

    Published: Jan 1991


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    Abstract

    The results of this study strongly indicate the practicability of using the streptavidin-biotin/nitrocellulose-enzymeimmunoassay (SAB/NC-EIA) procedure for the rapid and highly sensitive detection of recovered human enteroviruses from contaminated water. This procedure can serve as a valuable adjunct to infectivity procedures which are slow, highly complicated, and expensive. While cell culture assay can only detect the total amount of infectious virus, the SAB/NC-EIA method can detect the total amount of viral antigen present independent of its biological activity. If the water samples contain relatively larger amounts of viral antigen than infectious virus, the highly sensitive SAB/NC-EIA can be a valuable addition to viral-monitoring procedures. Other advantages are (a) because of its rapidity and sensitivity, the procedure can serve as an early-warning indicator of the presence of viral pathogens in all kinds of water; (b) the test is relatively easy to perform and the results can be read by visual observation, which eliminates the need for sophisticated instrumentation. Furthermore, quantitation of positive discs can be made by absorbance measurement made in an EIA plate reader or by analysis of transmission or reflectance densitometry; (c) lastly, the procedure can make the screening of viruses economical, reliable, sensitive, and rapid. This method would be convenient for microbiological laboratories with limited trained personnel and resources.

    Keywords:

    human enteroviruses, water, streptavidin/nitrocelluose-immunoassay, detection


    Author Information:

    Loh, PC
    Professor and ChairmanAssociate and Director, Water Resources Research Center, University of Hawaii, Honolulu, HI

    Fujioka, RS
    Professor and ChairmanAssociate and Director, Water Resources Research Center, University of Hawaii, Honolulu, HI

    Lau, LS
    Professor and ChairmanAssociate and Director, Water Resources Research Center, University of Hawaii, Honolulu, HI


    Paper ID: STP17622S

    Committee/Subcommittee: D19.24

    DOI: 10.1520/STP17622S


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