STP1102: Evaluation of a Method to Detect Giardia and Cryptosporidium in Water

    LeChevallier, MW
    Research MicrobiologistResearch TechnicianDirector, American Water Works Service Company Inc., Belleville LaboratoryResearch for the American Water Works Service Company Inc., BellevilleVoorhees, ILNJ

    Norton, WD
    Research MicrobiologistResearch TechnicianDirector, American Water Works Service Company Inc., Belleville LaboratoryResearch for the American Water Works Service Company Inc., BellevilleVoorhees, ILNJ

    Lee, RG
    Research MicrobiologistResearch TechnicianDirector, American Water Works Service Company Inc., Belleville LaboratoryResearch for the American Water Works Service Company Inc., BellevilleVoorhees, ILNJ

    Pages: 16    Published: Jan 1991


    Abstract

    The current study outlines a technique to simultaneously detect Giardia and Cryptosporidium in raw and tap water samples. The influence of various procedural steps, including filtration, elution, flotation, filter mounting and microscopy, was determined for the method. Overall, the method recovered 48% to 69% of the Giardia and 25% to 42% of the Cryptosporidium in spiked water samples. The method had an averaged detection limit of 1 organism/100 L of tap water. Use of phase-contrast and Nomarski differential interference contrast microscopy could detect false-positive algal cells and determine cysts and oocysts having “non-viable type” morphologies.

    Keywords:

    Giardia, Cryptosporidium, immunofluorescence techniques, phase-contrast microscopy, Nomarski differential interference contrast microscopy, parasite methods, surface water, drinking water


    Paper ID: STP17618S

    Committee/Subcommittee: D19.24

    DOI: 10.1520/STP17618S


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