STP1260: Validation Studies in the Regeneration of Ion-Exchange Celluloses

    Levison, PR
    Technical Manager (Media), Whatman International Ltd., Springfield Mill, Maidstone, Kent

    Streater, M
    Product Development Manager, Whatman International Ltd., Springfield Mill, Maidstone, Kent

    Jones, RMH
    Sales Specialist (Media), Whatman International Ltd., Springfield Mill, Maidstone, Kent

    Pathirana, ND
    Senior Research Technologist, Whatman International Ltd., Springfield Mill, Maidstone, Kent

    Pages: 12    Published: Jan 1996


    Abstract

    A clean-in-place procedure involving storage in 0.5M-NaOH for several hours has been found effective in the regeneration of process-scale columns (25 1) of Express-Ion ion-exchange celluloses following preparative loadings (2–9 kg) of a hen egg-white protein feedstock. These conditions have been demonstrated to provide simultaneous sanitization of the media following a gross microbial challenge, as indicated by decreased bioburden and endotoxin levels. We have developed an ion chromatography procedure for detecting and quantitating potential hydrolysis products originating from the functional groups bonded to anion and cation-exchange media. Using these techniques we demonstrate no detectable leakage of functional groups from the ion-exchange celluloses during the bed regeneration process. There was no significant loss of glucose from the matrix during this procedure.

    Keywords:

    ion-exchange, cellulose, CIP, sanitization, leachables, hen egg-white


    Paper ID: STP14709S

    Committee/Subcommittee: E48.03

    DOI: 10.1520/STP14709S


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