STP1317

    Macrophage Aggregates: Biomarker for Immune Function in Fishes?

    Published: Jan 1997


      Format Pages Price  
    PDF (284K) 16 $25   ADD TO CART
    Complete Source PDF (9.6M) 16 $118   ADD TO CART


    Abstract

    Macrophage aggregates (MAs) are believed to be functional equivalents of germinal centers, active in storage of exogenous and endogenous waste products, the immune response, and iron storage and recycling. Numerous studies have shown an increase in their number, size or hemosiderin content in fish collected at contaminated sites. For this reason, MAs have been suggested as potentially sensitive biomarkers of contaminant exposure. Although they are structures observed histologically, it has also been suggested they may be immunotoxicologic biomarkers. To determine possible relationships between MA formation and macrophage function, we examined data from two field studies and one laboratory study. We found a significant correlation between a decreased chemotaxic response of macrophages and the formation of more numerous, smaller MAs in mummichog from a contaminated site. In laboratory-exposures to arsenic, macrophage function appeared to be a more sensitive indicator at the lower levels of dietary arsenic. However, MA appeared to provide a more dose-dependent and comprehensive indicator of toxicity.

    Keywords:

    fish, macrophage aggregates, macrophage function, chemotaxis, polycyclic aromatic hydrocarbons, arsenic


    Author Information:

    Blazer, VS
    National Biological Service, Leetown Science Center, National Fish Health Research Laboratory, Kearneysville, WV

    Fournie, JW
    U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Breeze, FL

    Weeks-Perkins, BA
    College of Veterinary Medicine, North Carolina State University, Raleigh, NC


    Paper ID: STP12249S

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP12249S


    CrossRef ASTM International is a member of CrossRef.