STP1333: Identification of Cytochrome P4501A Inducers in Complex Mixtures of Polycyclic Aromatic Hydrocarbons

    Villeneuve, DL
    Research Assistant, Michigan State University, Dept. of Zoology, Institute for Environmental Toxicology, and Pesticide Research Center, East Lansing, MI

    De Vita, WM
    Research Specialist and Associate Professor of Water Resources, University of Wisconsin-Stevens Point, College of Natural Resources, Stevens Point, WI

    Crunkilton, RL
    Research Specialist and Associate Professor of Water Resources, University of Wisconsin-Stevens Point, College of Natural Resources, Stevens Point, WI

    Pages: 13    Published: Jan 1998


    Abstract

    An in vitro ethoxyresorufin O-deethylase (EROD) assay was used to study the ability of individual polycyclic aromatic hydrocarbons (PAHs) and mixtures of PAHs to induce Ah receptor (AhR) mediated cytochrome P4501A activity in PLHC-1 fish hepatoma cells. The purpose was to identify the most potent inducers from a set of thirteen separate PAHs and describe interactions occurring in complex mixtures of these PAHs. Where possible, potency was expressed in terms of 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TCDD-EQ) by normalizing the PAH results to a TCDD standard curve. The most potent inducers were benzo(k)fluoranthene > benzo(a) pyrene ≈ benzo(b)fluoranthene > chrysene ≈ benzo(a)anthracene. At equal concentrations, these PAHs yielded potencies of 1670, 940, 655, 255, and 185 pg TCDD-EQ/g, respectively. Analysis of various mixtures of the thirteen PAHs suggested that complex interactions may be occurring.

    Keywords:

    polycyclic aromatic hydrocarbons, EROD, TEF, PLHC-1, in vitro, bioassay, interactions, cytochrome P4501A (CYP1 A)


    Paper ID: STP12157S

    Committee/Subcommittee: E47.04

    DOI: 10.1520/STP12157S


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